Protein preparation, crystallization and preliminary X-ray analysis of human adrenal gland protein AD-004.

نویسندگان

  • Hui Ren
  • Yuhe Liang
  • Rui Li
  • Haitao Ding
  • Shihong Qiu
  • Shanyun Lu
  • Jianli An
  • Lanfen Li
  • Ming Luo
  • Xiaofeng Zheng
  • Xiao Dong Su
چکیده

The adrenal gland protein AD-004 was identified in the human adrenal gland. Full-length AD-004 contains 172 amino acids, with a predicted molecular weight of about 20 kDa. In attempts to crystallize human AD-004, the gene was subcloned into a modified pET vector, pET21-DEST, with an N-terminal His(5) tag using the Gateway cloning system, followed by protein expression in Escherichia coli strain BL21(DE3). The protein was purified in two steps to near-homogeneity and was crystallized. The crystals belong to space group P6(1) or P6(5), with unit-cell parameters a = b = 99.56, c = 57.19 A. A complete 2.0 A data set has been collected at a rotating-anode X-ray source and structure determination is under way.

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عنوان ژورنال:
  • Acta crystallographica. Section D, Biological crystallography

دوره 60 Pt 7  شماره 

صفحات  -

تاریخ انتشار 2004